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I'm doing an esterase assay on carbonic anhydrase II with 4-nitrophenyl acetate and fitting Michaelis–Menten in Prism. My highest substrate concentration was 3.6 mM, and it returns Km ≈ 9 mM — but the curve is clearly still below saturation. Is that Km reliable, or unusable because I never reached saturation? New to kinetics, thanks. submitted by /u/varkesurelis |
A complete quantum metrology theory for N-mode bosonic interferometers, founded on the Sp(2N, R) symmetry, now exists. Chenwei Lv and Renbao Liu at the Chinese University of Hong Kong have established this theory, where 2N defines the size of a key symmetric matrix. The theory describes how multimode quantum interferometers function, devices employing multiple beams…
A new study, led by experts at the University of Nottingham, suggests a pet gecko with an unusually high risk of tumors may be a promising model for understanding how cancer develops and spreads. The findings of the study, which are published in BMC Biology, could help to explain why some animals frequently get cancer…
Lance Whitney/ZDNET Follow ZDNET: Add us as a preferred source on Google. ZDNET’s key takeaways Ookla’s study measured speed and consistency among US telecom providers. T-Mobile was tops among the three major US mobile carriers. AT&T Fiber ranked first among major internet providers. Not happy with your current mobile carrier or internet provider and looking…
hi i have an old surplus aluminum bottle and i cleaned the inside of the bottle well, i have rinsed the bottle many times and I tried keeping regular tap water in it for about a day but the water starts to bubble a little and small lumps form on the inside of the bottle….
But how does lactase get this useful shape? It is encoded into the sequence of amino acids that make it up. As the chain is synthesized in the cell, thermal fluctuations allow the chain to explore its energy landscape and crumple up into its most stable equilibrium state. This process is a kind of thermodynamic…
Astronomers have directly confirmed four previously hidden white dwarf stars in nearby binary systems. Every system lies within 65 light-years of Earth, and one contains the ninth closest known white dwarf to the Sun. The discoveries were made by researchers at the University of Warwick and the University of Colorado Boulder. Their findings were published…
